Studies on Human Proinsulin
نویسندگان
چکیده
منابع مشابه
Studies on human proinsulin. Isolation and amino acid sequence of the human pancreatic C-peptide.
Human proinsulin C-peptide has been extracted from human pancreas with acid-ethanol and purified by means of gel filtration, carboxymethyl-cellulose chromatography, paper electrophoresis, and partition chromatography. The purified material was judged to be about 98% pure by compositional analysis. Amino acid sequence analysis was carried out on the intact C-peptide and fragments derived by hydr...
متن کاملHuman serum proinsulin.
Gel filtration of human serum extracts on Bio-Gel P-30 columns produced two peaks of material reactive with insulin antisera. The earlier eluting fraction appeared at the elution position of proinsulin (serum proinsulin-like component, PLC) while the second fraction corresponded in elution volume to insulin. In assays using porcine insulin-(131)I and an antiserum against porcine insulin, human ...
متن کاملStudies on the biological activity of porcine proinsulin.
The biological activity of purified porcine proinsulin was investigated in rats. In vivo studies revealed that proinsulin produced a hypoglycemic response similar to insulin but of lesser magnitude. Hypophysectomized and adrenalectomized animals proved to be more sensitive to proinsulin than normal. In vitro studies with rat hemidiaphragm were consistent with the in vivo findings. No competitio...
متن کاملRecombinant Human Proinsulin from Transgenic Corn Endosperm: Solvent Screening and Extraction Studies
Recombinant pharmaceutical proteins are being produced in different systems such as bacteria and mammalian cell cultures. The use of transgenic plants as bioreactors has recently arisen as an alternative system offering many practical and economic advantages. However, finding an optimum strategy for the downstream processing (DSP) of recombinant proteins from plants still remains a challenge. I...
متن کاملKinetics of proinsulin conversion in human islets.
Islets isolated from human cadaver pancreas were pulse-labeled (10 min with [3H]leucine) and then incubated for a 180-min chase. Islets and chase medium were collected every 15 min and analyzed by reversed-phase HPLC to quantify the percentage of radioactively labeled proinsulin, conversion intermediates, and fully processed insulin. Release of proinsulin-related labeled products into the chase...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1971
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)76984-1